Composite
Part:BBa_K4343127
Designed by: Zheng Zhe Group: iGEM22_NNU-China (2022-10-09)
TEFin-nCBE
A cytosine base editor with TEFin promoter-driven expression in which the Cas9 protein is nCas9. This system creates a gap at the targeting site and facilitates the efficiency of base editing in Y.lipolytica. The editing efficiency under this promoter has also been further improved, up to 85 %.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1538
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 4862
Illegal BamHI site found at 3817 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 426
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1
Illegal BsaI site found at 429
Illegal BsaI site found at 5440
Illegal BsaI.rc site found at 423
Illegal BsaI.rc site found at 4549
Illegal BsaI.rc site found at 5704
[edit]
Categories
Parameters
None |